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Image Search Results
Journal: BMC Cell Biology
Article Title: Assessment of ORAI1-mediated basal calcium influx in mammary epithelial cells
doi: 10.1186/1471-2121-14-57
Figure Lengend Snippet: HC11 cell differentiation and Ca 2+ influx. Total mRNA was isolated and assessed for an increase in β-casein mRNA to confirm differentiation using real-time RT-PCR. Calcium assays were conducted at the same time to measure changes in basal Ca 2+ influx in response to differentiation. A β-Casein mRNA levels increased in differentiated HC11 cells in comparison to proliferating cells. Data were normalized to Ppib and Actb mRNA, and are shown as relative fold expression to differentiated HC11 cells (n = 4; mean ± SD, * P < 0.05). B Basal Ca 2+ influx is shown as a trace of mean fluorescence relative to baseline in proliferating and differentiated HC11 cells. C Rate of basal Ca 2+ influx, calculated as the slope between 0–5 s after 2 mM Ca 2+ addition, in differentiated and proliferating HC11 cells. Rate is expressed as a percentage relative to differentiated cells treated with siNT and CPA (n = 4; mean ± SEM, * P < 0.05).
Article Snippet: Calcium assays were performed using a fluorometric imaging plate reader (FLIPR TETRA , Molecular Devices Corporation) using the
Techniques: Cell Differentiation, Isolation, Quantitative RT-PCR, Expressing, Fluorescence
Journal: BMC Cell Biology
Article Title: Assessment of ORAI1-mediated basal calcium influx in mammary epithelial cells
doi: 10.1186/1471-2121-14-57
Figure Lengend Snippet: Effect of Orai1 silencing on basal Ca 2+ in HC11 cells. Calcium assays were conducted to assess basal Ca 2+ influx in proliferating and differentiated HC11 cells treated with siNT or siOrai1. A Basal Ca 2+ influx is shown as a trace of mean fluorescence relative to baseline in proliferating and differentiated HC11 cells treated with siNT and siOrai1. B Rate of basal Ca 2+ influx, calculated as the slope between 0–5 s after 2 mM Ca 2+ addition, in differentiated and proliferating HC11 cells with siNT or siOrai1. Rate is expressed as a percentage relative to differentiated cells treated with siNT and CPA (n = 4; mean ± SEM, * P < 0.05).
Article Snippet: Calcium assays were performed using a fluorometric imaging plate reader (FLIPR TETRA , Molecular Devices Corporation) using the
Techniques: Fluorescence
Journal: BMC Cell Biology
Article Title: Assessment of ORAI1-mediated basal calcium influx in mammary epithelial cells
doi: 10.1186/1471-2121-14-57
Figure Lengend Snippet: Effect of Stim1 silencing on basal Ca 2+ in HC11 cells. Calcium assays were conducted to assess the basal Ca 2+ influx in proliferating and differentiated HC11 cells treated with siNT or siStim1. A Basal Ca 2+ influx is shown as a trace of mean fluorescence relative to baseline in proliferating and differentiated HC11 cells treated with siNT and siStim1. B Rate of basal Ca 2+ influx, calculated as the slope between 0–5 s after 2 mM Ca 2+ addition, in differentiated and proliferating HC11 cells with siNT or siStim1. Rate is expressed as a percentage relative to differentiated cells treated with siNT and CPA (n = 4; mean ± SEM, * P < 0.05).
Article Snippet: Calcium assays were performed using a fluorometric imaging plate reader (FLIPR TETRA , Molecular Devices Corporation) using the
Techniques: Fluorescence
Journal: BMC Cell Biology
Article Title: Assessment of ORAI1-mediated basal calcium influx in mammary epithelial cells
doi: 10.1186/1471-2121-14-57
Figure Lengend Snippet: Effect of Stim2 silencing on basal Ca 2+ in HC11 cells. Calcium assays were conducted to assess the basal Ca 2+ influx in proliferating and differentiated HC11 cells treated with siNT or siStim2. A Basal Ca 2+ influx is shown as a trace of mean fluorescence relative to baseline in proliferating and differentiated HC11 cells treated with siNT and siStim2. B Rate of basal Ca 2+ influx, calculated as the slope between 0–5 s after 2 mM Ca 2+ addition, in differentiated and proliferating HC11 cells with siNT or siStim2. Rate is expressed as a percentage relative to differentiated cells treated with siNT and CPA (n = 4; mean ± SEM, * P < 0.05).
Article Snippet: Calcium assays were performed using a fluorometric imaging plate reader (FLIPR TETRA , Molecular Devices Corporation) using the
Techniques: Fluorescence
Journal: BMC Cell Biology
Article Title: Assessment of ORAI1-mediated basal calcium influx in mammary epithelial cells
doi: 10.1186/1471-2121-14-57
Figure Lengend Snippet: Effect of Spca2 silencing on basal Ca 2+ in HC11 cells. Calcium assays were conducted to assess the basal Ca 2+ influx in proliferating and differentiated HC11 cells treated with siNT or siSpca2. A Basal Ca 2+ influx is shown as a trace of mean fluorescence relative to baseline in proliferating and differentiated HC11 cells treated with siNT and siSPCA2. B Rate of basal Ca 2+ influx, calculated as the slope between 0–5 s after 2 mM Ca 2+ addition, in differentiated and proliferating HC11 cells with siNT or siSpca2. Rate is expressed as a percentage relative to differentiated cells treated with siNT and CPA (n = 4; mean ± SD, * P < 0.05).
Article Snippet: Calcium assays were performed using a fluorometric imaging plate reader (FLIPR TETRA , Molecular Devices Corporation) using the
Techniques: Fluorescence